flow pearson correlation analysis Search Results


gene exp acadvl hs00825606 g1  (Thermo Fisher)
87
Thermo Fisher gene exp acadvl hs00825606 g1
HSL overexpression is sufficient to drive a pro-resolving phenotype. Human M0 macrophages were transfected with an empty vector or an HSL-encoding plasmid. (A) Efferocytosis efficiency was measured 24 h post-transfection ( n = 5 independent donors). (B) Surface expression of M2-associated markers CD206, CD163, and CD36 (gMFI by flow cytometry) and secreted levels of Annexin A1 (ELISA, n = 4–6, independent donors). (C) Secreted levels of pro-inflammatory cytokines TNFα and IL-1β were measured by ELISA ( n = 6–7 independent donors). (D) Relative mRNA expression of the master regulator PPARγ and genes involved in fatty acid oxidation, <t>ACADVL</t> and CPT1a ( n = 5–6, independent donors). (E-F) HSL overexpression enhances mitochondrial respiratory capacity in macrophages. (E) A representative Seahorse XF Mito Stress test of macrophages transfected with a control vector (Vector, red line) or an HSL-encoding plasmid (HSL, blue line) is shown. The trace represents the mean ± SEM of three technical replicates from a single donor. OCR: oxygen consumption rate. 1.5 µM Oligomycin, 1 µM FCCP, 0.5 µM Rotenone + Antimycin A. (F) Basal respiration, maximal respiration, spare respiratory capacity, and ATP production were determined using the Seahorse XF Cell Mito Stress Test report generator. Each dot indicates the average from one of n = 6 independent donors. For all panels, a paired t -test was used. Data in panels A, B (Annexin A1) and C are presented as box-and-whisker plots and in panels F as mean ± SEM. Panels B & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant
Gene Exp Acadvl Hs00825606 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow pearson correlation analysis  (Partek)
86
Partek flow pearson correlation analysis
HSL overexpression is sufficient to drive a pro-resolving phenotype. Human M0 macrophages were transfected with an empty vector or an HSL-encoding plasmid. (A) Efferocytosis efficiency was measured 24 h post-transfection ( n = 5 independent donors). (B) Surface expression of M2-associated markers CD206, CD163, and CD36 (gMFI by flow cytometry) and secreted levels of Annexin A1 (ELISA, n = 4–6, independent donors). (C) Secreted levels of pro-inflammatory cytokines TNFα and IL-1β were measured by ELISA ( n = 6–7 independent donors). (D) Relative mRNA expression of the master regulator PPARγ and genes involved in fatty acid oxidation, <t>ACADVL</t> and CPT1a ( n = 5–6, independent donors). (E-F) HSL overexpression enhances mitochondrial respiratory capacity in macrophages. (E) A representative Seahorse XF Mito Stress test of macrophages transfected with a control vector (Vector, red line) or an HSL-encoding plasmid (HSL, blue line) is shown. The trace represents the mean ± SEM of three technical replicates from a single donor. OCR: oxygen consumption rate. 1.5 µM Oligomycin, 1 µM FCCP, 0.5 µM Rotenone + Antimycin A. (F) Basal respiration, maximal respiration, spare respiratory capacity, and ATP production were determined using the Seahorse XF Cell Mito Stress Test report generator. Each dot indicates the average from one of n = 6 independent donors. For all panels, a paired t -test was used. Data in panels A, B (Annexin A1) and C are presented as box-and-whisker plots and in panels F as mean ± SEM. Panels B & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant
Flow Pearson Correlation Analysis, supplied by Partek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow pearson correlation analysis/product/Partek
Average 86 stars, based on 1 article reviews
flow pearson correlation analysis - by Bioz Stars, 2026-06
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pearson's correlation coefficient  (SPSS Inc)
90
SPSS Inc pearson's correlation coefficient
HSL overexpression is sufficient to drive a pro-resolving phenotype. Human M0 macrophages were transfected with an empty vector or an HSL-encoding plasmid. (A) Efferocytosis efficiency was measured 24 h post-transfection ( n = 5 independent donors). (B) Surface expression of M2-associated markers CD206, CD163, and CD36 (gMFI by flow cytometry) and secreted levels of Annexin A1 (ELISA, n = 4–6, independent donors). (C) Secreted levels of pro-inflammatory cytokines TNFα and IL-1β were measured by ELISA ( n = 6–7 independent donors). (D) Relative mRNA expression of the master regulator PPARγ and genes involved in fatty acid oxidation, <t>ACADVL</t> and CPT1a ( n = 5–6, independent donors). (E-F) HSL overexpression enhances mitochondrial respiratory capacity in macrophages. (E) A representative Seahorse XF Mito Stress test of macrophages transfected with a control vector (Vector, red line) or an HSL-encoding plasmid (HSL, blue line) is shown. The trace represents the mean ± SEM of three technical replicates from a single donor. OCR: oxygen consumption rate. 1.5 µM Oligomycin, 1 µM FCCP, 0.5 µM Rotenone + Antimycin A. (F) Basal respiration, maximal respiration, spare respiratory capacity, and ATP production were determined using the Seahorse XF Cell Mito Stress Test report generator. Each dot indicates the average from one of n = 6 independent donors. For all panels, a paired t -test was used. Data in panels A, B (Annexin A1) and C are presented as box-and-whisker plots and in panels F as mean ± SEM. Panels B & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant
Pearson's Correlation Coefficient, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pearson's correlation coefficient/product/SPSS Inc
Average 90 stars, based on 1 article reviews
pearson's correlation coefficient - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


HSL overexpression is sufficient to drive a pro-resolving phenotype. Human M0 macrophages were transfected with an empty vector or an HSL-encoding plasmid. (A) Efferocytosis efficiency was measured 24 h post-transfection ( n = 5 independent donors). (B) Surface expression of M2-associated markers CD206, CD163, and CD36 (gMFI by flow cytometry) and secreted levels of Annexin A1 (ELISA, n = 4–6, independent donors). (C) Secreted levels of pro-inflammatory cytokines TNFα and IL-1β were measured by ELISA ( n = 6–7 independent donors). (D) Relative mRNA expression of the master regulator PPARγ and genes involved in fatty acid oxidation, ACADVL and CPT1a ( n = 5–6, independent donors). (E-F) HSL overexpression enhances mitochondrial respiratory capacity in macrophages. (E) A representative Seahorse XF Mito Stress test of macrophages transfected with a control vector (Vector, red line) or an HSL-encoding plasmid (HSL, blue line) is shown. The trace represents the mean ± SEM of three technical replicates from a single donor. OCR: oxygen consumption rate. 1.5 µM Oligomycin, 1 µM FCCP, 0.5 µM Rotenone + Antimycin A. (F) Basal respiration, maximal respiration, spare respiratory capacity, and ATP production were determined using the Seahorse XF Cell Mito Stress Test report generator. Each dot indicates the average from one of n = 6 independent donors. For all panels, a paired t -test was used. Data in panels A, B (Annexin A1) and C are presented as box-and-whisker plots and in panels F as mean ± SEM. Panels B & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant

Journal: Cell Communication and Signaling : CCS

Article Title: Hormone-sensitive lipase drives pro-resolving macrophage polarization and enhances efferocytosis

doi: 10.1186/s12964-025-02631-z

Figure Lengend Snippet: HSL overexpression is sufficient to drive a pro-resolving phenotype. Human M0 macrophages were transfected with an empty vector or an HSL-encoding plasmid. (A) Efferocytosis efficiency was measured 24 h post-transfection ( n = 5 independent donors). (B) Surface expression of M2-associated markers CD206, CD163, and CD36 (gMFI by flow cytometry) and secreted levels of Annexin A1 (ELISA, n = 4–6, independent donors). (C) Secreted levels of pro-inflammatory cytokines TNFα and IL-1β were measured by ELISA ( n = 6–7 independent donors). (D) Relative mRNA expression of the master regulator PPARγ and genes involved in fatty acid oxidation, ACADVL and CPT1a ( n = 5–6, independent donors). (E-F) HSL overexpression enhances mitochondrial respiratory capacity in macrophages. (E) A representative Seahorse XF Mito Stress test of macrophages transfected with a control vector (Vector, red line) or an HSL-encoding plasmid (HSL, blue line) is shown. The trace represents the mean ± SEM of three technical replicates from a single donor. OCR: oxygen consumption rate. 1.5 µM Oligomycin, 1 µM FCCP, 0.5 µM Rotenone + Antimycin A. (F) Basal respiration, maximal respiration, spare respiratory capacity, and ATP production were determined using the Seahorse XF Cell Mito Stress Test report generator. Each dot indicates the average from one of n = 6 independent donors. For all panels, a paired t -test was used. Data in panels A, B (Annexin A1) and C are presented as box-and-whisker plots and in panels F as mean ± SEM. Panels B & D show paired individual donors. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant

Article Snippet: Primers for IL1B (Hs01555410_m1), HIF1A (Hs00153153_m1), MERTK (Hs1031973_m1), CD206 /MRC1 (Hs00267207_m1), PPARG (Hs01115513_m1), ATGL/ PNPLA2 (Hs00982042_m1), LIPE (HSL, Hs00943410_m1), TNF (Hs00174128_m1), CD36 (Hs00354519_m1), CD200R1 (Hs00793597_m1), ASAH1 (Hs00924966_m1), ACADVL (Hs00825606_g1), CPT1A (Hs00912671_m1), CPT2 (Hs00988962_m1), SLC25A2 0/CACT (Hs00386383_m1), MFN2 (Hs00208382_m1), FABP3 (Hs07287863_m1), Annexin A1 (ANXA1, Hs00167549_m1) and HPRT1 (Hs99999909_m1) were obtained from Thermo Fisher Scientific (Waltham, MA).

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control, Whisker Assay